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Bound proteins were washed several times, then eluted from beads with Flag peptide at 200 g/ml in wash buffer (Sigma) and incubated with Strep-Tactin beads in wash buffer at 4 C overnight

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The neurotrophic agents capable of facilitating synaptogenesis and neurogenesis include nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3, and neurotrophin-4 [34,35]

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